Project

Part:BBa_K3113320

Designed by: Alejandro Salinas Illarena   Group: iGEM19_Munich   (2019-10-13)


pCAG_mGag-HiBit-DHD154a

This plasmid codes for the coat protein of gag fused to a split luciferase fused to a synthetic coiled-coil structure. This construct forms virus-like particles which can be detected via a split luciferase assay. Through the coiled-coil domain the vesicles can be loaded. The coat protein of HIV was modified to guarantee budding in Mus musculus.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2491
    Illegal BglII site found at 3019
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2421
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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